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Galleria mellonella
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How to Build a Mitotic Spindle
Daniel Hayward, & James G Wakefield
Supplementary movies
Spindles usually form in an "inwards-out manner"
Cold treatment of mitotic Drosophila embryos reveals a chromatin mediated MT nucleation pathway
D-TPX2 and D-HURP are both Drosophila homologues of vertebrate SAFs, with shorter spindles forming in their absence
D-TPX2 and D-HURP are both mediators of chromatin mediated MT nucleation
Anti-Dgt6 interfering antibody inject is able to disrupt Augmin, resulting in long weak spindles
When Augmin is perturbed, pro-metaphase MT nucleation is significantly reduced
Augmin is required for chromatin mediated MT nucleation
D-HURP and Augmin have distinct roles
At high levels, anti-Spd2 breaks apart centrosomes resulting in artificial aMTOCs
Lower levels of anti-Spd2 injection following cold treatment reduce astral input into spindle assembly
Spindles fail to form when asters and Augmin are perturbed
Home
Research
Mitosis
Galleria mellonella
Current Lab Members
Past Lab Members (2010-)
Pictures of the Group
Publications
Movies and Images
Drosophila Links
MS
Lab Blog
Contact Us